dna shuffling meaning in English
有性pcr
Examples
- The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration . the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology . - amylase ( ec 3 . 2 . 1 . 1 ; 1 , 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1 , 4 - glycosidic bonds of starch from middle and liberates - maltose , - glucose and - limit dextrin stepwise
本试验根据genbank已公布的黄单胞菌-淀粉酶基因的核苷酸序列由引物设计软件premierprimer5 . 0辅助设计了一对引物( primer & primer ) ，以pbluescript ks +和puc18 / puc19质粒为载体，用常规的pcr方法从xanthomonascampestrispv . malvacearum ( smith ) dye等七株黄单胞菌( xanthomomasspp . )的基因组dna中克隆得到8个基因片段，分别命名为zhyf001 zhyf008 。 - Therefore , a - amylase has been used widely in many industrial fields , such as glucose production , beer brewing , fermentation trade , textile industry , and so on . the study on a - amylase is one of the most active fields in enzyme industrial fields . with the development of biotechnology , more and more scientists and researchers attempt to use dna shuffling technology to breed , screen and even " create " new a - amylase genes with higher activity and other new characters
设计引物时，上下游引物5 ’端分别添加了kpn和bamh酶切位点，克隆得到的基因片段和质粒载体都用kpn和bamh进行双酶切后，进行酶连、转化、筛选得到阳性重组子，经过蓝白斑验证、单酶切验证、双酶切验证、 pcr验证等一系列的验证后进行测序。 - It clearly allows us to engineer enzymes with novel functions and features by strategies , such as error - prone pcr , dna shuffling and stagger extension process ( step ) , when very little is known about spatial configuration of proteins . therefore millions of years in nature can in principle be accomplished in the test within several years . according to the basic idea of directed evolution , we modified the a - aldc gene by error - prone pcr
它不需要事先了解酶的空间结构和催化机制，而通过在体外模拟自然进化机制(随机突变，重组和自然选择) ，利用易错pcr ， dna改组，交错延伸等方法，改造酶基因，定向选择(或筛选)有价值的非天然酶，短期内在试管中完成在自然界需要几百万年的进化过程。